![]() Oh, and for the record, if you’re really feeling randy, the bar makes good on its free-bottle offer, too. ![]() Place barspoon of grenadine over the top. US Glove Hot Shot Double Buckle grip for uneven bars has our same narrow Hot Shot design in durable, heavier leather. Topper Harley, jeden z najlepszych pilotów myliwców w Stanach na prob swoich przeoonych wraca do czynnej suby, aby wzi udzia w niezwykle tajnej operacji wojskowej. Combine rum, schnapps and juice in a cocktail shaker with ice. Excludes big shot burger and massive woody. ![]() If you end up in the buff, ask for the Gypsy Bitch, a mixture of coconut liqueur, peach schnapps, and vodka. Zwariowana komedia parodiujca najgoniejsze filmy ostatnich lat: Taczcy z wilkami, Top Gun, Dziewi i pó tygodnia, Superman etc. View Hotshots Sports Bar and Grill's June 2023 deals and menus. Sometimes referred to as Soi Yodsak or Soi Half a Dozen, it is one of those. Think the sign that says “Get Naked, Get A Free Shot” is a joke? The Bulgarian Bar actually makes good on the promise (two or three times a week, sometimes a couple of times a night, by the owner’s estimation). Pattaya Soi 6 is one of the more notorious parts of the city’s famous nightlife. Follow it up with something sweet: Cherry Bombs are maraschino cherries soaked in Bacardi 151 rum. The Black Bear Lodge’s Pepper F*cker is a punishing shot of Cuervo Gold that’s infused with jalapeño and five other types of peppers. 'Hot shots' may also refer to Ziggy and Nick, who. Burroughs s novel Junkie, a hot-shot is a portion of drugs that has been spiked with poison, usually with the intention of killing a police informant or other undesirable. At Cobble Hill’s Buck’s Lodge, the barkeep hollers “Buck … shot!” every time someone gets “bucked up” on a shot of whiskey infused with nutmeg and cinnamon (it’s best chased with upstate microbrew Buck’s Piss). The bad package brought into the prison is referred to as 'hot shots' by an inmate. Something about a cabin-themed bar demands a bracing shot. Then, keeping to the theme, we found a boutique bar in Kings Cross and hit up the martinis and gin and tonics for a spot of false confidence. The Buck Shot at Buck’s Lodge and the Pepper F*cker at Black Bear LodgeĤ5 Atlantic Ave., nr.
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![]() Preceden is free to try with paid plans starting at $12/month. Since it's completely web-based, Preceden is compatible with both Mac and PC computers – moreover, no download is required to use this tool. Sure, this may be a bit biased, but we believe Preceden is the best timeline software on the net regardless of whether you're using it for project management, historical timelines, or even genealogy research. With its intuitive web-based editor for creating interactive timelines, timeline templates, and numerous export options you'll be able to build and share your timeline in no time at all. Preceden is an easy-to-use timeline maker and roadmapping tool that helps you quickly create professional timelines. You can use the following filter to drill down based on your requirements: If you're short on time, here are the top tools you should check out:Īnd if you have some more time, check out the our complete list of 28 timeline makers below. Top 10 Rated Tools for Creating Timelines in 2023 ![]() If several tools will get the job done, the strength of their support docs and quality of their support response could be a deciding factor. Some may have been neglected for years and have poor support (you can send an email to their support team to see how quickly they respond). In addition to the differing features, pricing, and styles, be sure to check out other aspects of each offering. If you need a paid plan (which will typically unlock additional features) you'll want to make sure to choose a tool in your budget, which may be drastically different depending on whether the tool is for students or a project managers at Fortune 100 companies. What can I afford? Most tools, including ours ( Preceden), provide a free plan or trial which may be more than enough for your timeline.A cartoon-looking timelines might be fine if you're working on a homework assignment but may not work well if you'll be presenting the timeline to a board of directors. What style do I prefer? Every tool creates a different style of timeline.What platforms does the tool support? Some tools are web-based and can be used from any device whereas some only work on Windows or Macs. ![]()
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The latest version of the Opera browser sends multiple invalid requests to our servers for every page you visit.The most common causes of this issue are: if you interest to watch Bird of Steel if yes, you come in the right site, today you can stream Bird of Steel (2016) for without any cost with full Movie, Bird of Steel is a perfect movie in 2016, now you can stream Bird of Steel (2016) in this web without any cost with full Bluray movie Rate: 6. Continued abuse of our services will cause your IP address to be blocked indefinitely.Your IP address has been temporarily blocked due to a large number of HTTP requests. ![]() ![]() Using GameFAQs regularly with these browsers can cause temporary and even permanent IP blocks due to these additional requests. The most common causes of this issue are: Your IP address has been temporarily blocked due to a large number of HTTP requests. ![]() Join the Slice Rewards program to earn free pizzas. However, you can use a Slice promo code with a Pizza Points redemption offer. You can’t combine Slice coupon codes or stack multiple coupons on a single purchase. If you still have trouble, try a different Slice discount code from CouponFollow instead. Make certain the coupon which you selected is valid and has not yet expired. Verify that you have correctly input your Slice discount code.Įnsure your cart meets the minimum purchase amount or any other requirements. What To Do if Your Slice Coupon Code Isn't Working You can provide your contact information and billing details on the following screen.Ĭlick to expand the “Add promo code” field you’ll see a link right below the “Payment” heading.Įnter your Slice promo code in the text field and click “Apply” to unlock your pizza discount. Log into your Slice account, or select “Skip” to check out as a guest. Enter your address, select a delivery option, and click “Get Pizza” to find your preferred local pizzeria.Ĭustomize your order with toppings and appetizers as desired, then click “Add to Cart” to place it in your bag.Ĭlick “Checkout” at the bottom of the “Your Order” side menu when your Slice order is complete. You can check current offers in the “Deepest Discounts” section of the Slice app or look for coupons and offers on the pizzeria search page. No, there isn’t a specific “deals” or “coupons” page at. Is there a coupon page on the Slice website? If your local pizzeria offers a student discount, you can’t use it on a Slice order. No, Slice doesn’t offer a student discount or any special deals for students. You’ll earn one Pizza Point for every order over $30 and a free large cheese pizza for every eight points.Ĭan I get a student discount at ? Download the Slice app and create an account to join. ![]() Yes, it's the free Slice Rewards program. Delivery fees typically range from $2 to $4. It depends, but Slice doesn’t determine delivery prices your local pizzeria sets its own delivery fees. Visit your account settings to enable email updates if you want to get Slice discount codes delivered to your inbox. Yes, Slice occasionally sends special offers and promotions to registered users. Some promo codes work sitewide, while others only apply to specific regions and restaurants. Yes, you can use Slice promo codes to save on your next pizza purchase, get free add-on items, and deduct delivery fees. Can I use a promo code on my Slice order? ![]() Boxers are moderate yearround shedders despite their lack of an undercoat. The Boxer’s coat is short, smooth, and glossy. A boxer’s topline is level, its neck relatively long and powerful, and the chest broad and deep.Īdult Boxers should be well-muscled through their bodies without appearing bulky or “buff.” When a boxer looks at you attentively is when you will see the wrinkles in her forehead. In fact, the AKC penalizes natural tails in sanctioned conformation shows, although it no longer requires cropped ears. In the US many owners crop their Boxer’s ears with a rather long trim that allows them to reflect the proud carriage of the dog’s head. The ears are medium-sized and high-set and forward when your dog is alert. A Boxer has a large head that still appears balanced with a broad, shortened muzzle and an undershot jaw. Even a larger individual should give the impression of being a medium-sized, compact, squarely built dog. Females can be significantly smaller than males. Physical Appearance of Boxersīoxers are medium to medium-large, standing 22 to 25 inches tall at the shoulders and weighing 50 to 80 pounds. The AKC had accepted the late-blooming Boxer in 1904. ![]() Some experts contend the Boxer was the result of last-ditch efforts to reinstate the “bull biters.” Boxer puppies for sale gained popularity in the US with a show dog called Bang Away in the 1950s, and the breed never looked back. Like the Old English Bulldog, the Bullenbeisser became extinct in the early 1900s through crossbreeding. Bullenbeissers were strong, agile, versatile dogs used for hunting, guarding, bull-baiting, dog fighting, and catching livestock. Boxers developed from the smaller dogs or Brabanter Bullenbeissers. Smaller dogs were 40 to 60 pounds, and the larger ones were 70 to 100 pounds. Thought to have arrived from Asia around 370 AD, two lines eventually emerged in Europe. Bullenbeissers are now extinct but resembled Old English Bulldogs. Originally the Boxer was a cross between a Bullenbeisser, or German Bulldog, and various other breeds such as the Great Dane, English Bulldog, sundry terriers, and the Mastiff. Boxer Puppies for Sale About the Boxer Historyīoxers came from Germany. Boxers thrive with owners who are active, adventurous, and willing to carve out plenty of activities for a boisterous dog. With its flashy looks, athletic abilities, and winning personality, a Boxer makes a good companion for single people, couples, or families with children over nine years old. He has participated in the military, the police force, hunting, cattle driving, guiding the blind, and guarding the home. ![]() The Boxer is both elegant and dignified yet not above silly antics. Few dogs can pull off acting like a clown and looking intimidating at the same time. Having one of the most lovable faces, the Boxer wears many hats. Boxer puppies for $300 Boxer puppies for $500 Boxer puppies for sale under $300 Boxer puppies for sale near me under $500 Boxer puppies for sale under $1,000 Flash, Brawn, and Personality: Boxer Puppies for Sale Have it All ![]()
The English translation is, so far it looks like sunscreens with nanoparticles do stay on the surface of the skin where they should be.Īll in all, Titanium Dioxide is a famous sunscreen agent and for good reason, it gives broad spectrum UV protection (best at UVB and UVA II), it's highly stable, and it has a good safety profile. ![]() A 2009 review article about the safety of nanoparticles summarizes this, "to date, in-vivo and in-vitro studies have not demonstrated percutaneous penetration of nanosized particles in titanium dioxide and zinc oxide sunscreens". But do not panic, these are concerns under investigation. Once absorbed they might form unwanted complexes with proteins and they might promote the formation of evil free radicals. The main concern with nanoparticles is that they are so tiny that they are absorbed into the skin more than we want them (ideally sunscreen should remain on the surface of the skin). The itsy-bitsy Nano-sized particles improve both spreadability and reduce the whitish tint a lot, but unfortunately, it also introduces new health concerns. The cosmetic industry is, of course, really trying to solve this problem and the best solution so far is using nanoparticles. Sunscreens containing Titanium Dioxide are often hard to spread on the skin and they leave a disturbing whitish tint. The disadvantage of Titanium Dioxide is that it's not cosmetically elegant, meaning it's a white, "unspreadable" mess. Regular sized Titanium Dioxide also has a great safety profile, it's non-irritating and is pretty much free from any health concerns (like estrogenic effect worries with some chemical filters). ![]() Its protection is very good between 290 - 350 nm (UVB and UVA II range), and less good at 350-400 nm (UVA I) range. Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate CopolymerĪnyway, it doesn't matter if it reflects or absorbs, Titanium Dioxide is a pretty awesome sunscreen agent for two main reasons: it gives a nice broad spectrum coverage and it's highly stable. ![]() You can also tell ip to only show you IPv4 information for a given network interface. So it's better to use the command ip in these situations.įor example: $ ip addr show wlan0 | grep -Po 'inet \K+' Solutions (using ip)Īs was also mentioned in the comments, ifconfig can be troublesome to use on systems that have multiple IP addresses assigned to a network device, given it only returns the first one. Currently I make the user set the IP address. I am currently using docker-compose to create a local version of my full deployment, however I cant access it using localhost or 127.0.0.1, I have to refer to the current machines IP address, for example curl 192.168.0.23:80. This solution makes use of grep's ability in newer versions to make use of PCRE (Perl regular expressions), along with it's -o switch to return just what matches the regular expression. I need to get the IP address the same way for Windows Linux and Mac OS. The third command will return the owner's preferred address, which may either an IPv4 or an IPv6 address. The second command returns the resolved IPv6 address. The first command returns the resolved IPv4 address. If the host points to a CNAME it will also get the IP in that case. demonstrated that there is an even more compact version using grep: $ ifconfig wlan0|grep -Po 't addr:\K+' All commands will resolve an IP address if the host still exists. There are countless other ways to do this, these are just a couple of examples to get you started. ![]() The Perl example is about as compact as it can get. You could also do it using perl: $ ifconfig wlan0 | perl -nle'/t addr:(\S+)/&print$1' You could for example use this awk script to parse out the IP address of your wireless LAN NIC (wlan0): $ ifconfig wlan0 | grep "inet " | awk -F'+' '' The commands below explicitly ignore this, but could be adapted quite easily to grab this information instead. If your system is configured to support IPv6 you'll see both the "dotted quad" as well as the IPv6 IP addresses in the ifconfig output.įor example: inet6 addr: fe80::226:c7ff:fe85:a720/64 Scope:Link For example if it's a wired NIC card, then it's likely eth0.Īlso these examples are returning the IPv4 address. I have a text file named abd shown below. Adjust this bit in the examples for your particular situation. The examples below assume that the network interface is a wireless card named wlan0. ![]() Next, we explored chromosome-associated RNAs in mitotic cells by isolating RNA from both the NETN100 soluble fraction and the pellets with TRIzol (Fig. In the pellets but not the NETN100 soluble fraction, we found nucleosomal histones, suggesting that components tightly associated with condensed chromosomes exist in the pellets, in agreement with earlier studies 19, 20. ![]() S1d), indicating that the NETN100 soluble fraction represents cytoplasmic/nucleoplasmic fraction of mitotic cells. In contrast, we did not find these representative proteins in the pellet fraction (Supplementary Fig. With western blotting, we detected cytoplasmic and nucleoplasmic proteins, including RP元 and GADPH, in the NETN100 soluble fraction. Moreover, we performed mitotic spreads and did not observe any interphase cells (Supplementary Fig. We found that all the cells were arrested at 4N stage, and majorities of cells were stained positively with anti-histone H3pSer10 antibody (Supplementary Fig. To validate the mitotic population, cells were examined by flow cytometry with Propidium Iodide and anti-histone H3pSer10 antibody. Mitotic cells were shaken off and lysed with NETN100 buffer (0.5% NP-40, 50 mM Tris-HCl, pH 8.0, 2 mM EDTA, and 100 mM NaCl), and both the NETN100 soluble fraction and the pellets were harvested (Fig. To examine the chromosome-associated RNAs, we pretreated HCT116 cells with colcemid to arrest cells in prometaphase (Fig. RRNAs are associated with mitotic chromosomes These chromosome-associated rRNAs facilitate chromosome dispersion during prometaphase. These rRNA species are evenly distributed in the perichromosomal layer of each chromosome via the interaction with Ki-67. In this study, we have carefully examined the chromosome-associated RNAs, and found that rRNAs are the major RNA species associating with condensed chromosomes. ![]() Since nucleolar proteins associate with small nucleolar RNA (snoRNA) to form RNPs, some snoRNA species, such as U3 snoRNA, are reported to be present at chromosome periphery 7, 17, 18. In contrast to the protein components, mitotic chromosome-associated RNA species are much less studied. It has been shown that the C-terminal Leu-Arg rich (LR) domain recognizes chromosomal DNA and anchors Ki-67 on mitotic chromosomes, whereas the other regions of Ki-67 extend outwards to form the perichromosomal layer 16. Ki-67 is a large nuclear polypeptide with several prominent domains. It has been shown that loss of Ki-67 depletes the mass of the perichromosomal layer and causes the coalescing of mitotic chromosomes 8, 16, suggesting that Ki-67 plays a key role to maintain the integrity of the perichromosomal layer. Interestingly, Ki-67 is released before nucleolus disassembly and relocates to the perichromosomal layer of condensed chromosomes 13. During mitotic prophase, the nucleolus is disassembled along with nuclear envelope breakdown 15. Although Ki-67 may be involved in ribosomal biogenesis 11, 12, 13, it is dispensable for the generation of mature rRNAs 14. Ki-67, a surrogate marker for cell proliferation, localizes in the nucleolus during interphase. Proteomics analysis shows that a number of proteins associate with the perichromosomal layer, and one prominent protein is Ki-67 9, 10. A recent analysis combining scanning electron microscopy with advanced proteomics indicates that the perichromosomal layer comprises 30%–47% of the entire chromosome volume 8. The outer layer of condensed chromosomes, also known as the perichromosomal layer, includes a number of proteins and RNA species 7. The inner scaffold contains proteins, such as condensin, for maintaining the integrity of the condensed chromosomes 5, 6. Mitotic chromosomes are composed of several specific regions. It is unclear whether the identities of chromatin-associated RNAs are altered during the chromatin condensation process. During mitotic prophase, chromatin undergoes condensation and forms highly compacted chromosomes. Chromatin-associated RNAs play key roles in regulating numerous biological events, such as chromatin remodeling and transcription 1, 2, 3, 4. ![]() Liszt may have been cavalier (my opinion -author Oliver Holmes does not interpret) in his after concert trysts for instance, he used Chopin’s apartment to seduce Chopin’s former fiancée and who was the current wife of his friend. They threw bouquets, fought (physically) for souvenirs such as his handkerchief, they grabbed and smoked his cigar butt, they embroidered his portrait, they wore gloves and broaches bearing his likeness, they framed upholstery fabric from chairs where he allegedly sat and, flirted and slept with him. Women swooned for him as they would do in the future for Valentino, Sinatra, Elvis, the Beatles and Justin Bieber. This time, as an eligible young man, he developed a sensual stage presence. After a quiet period of supporting his mother by giving piano lessons in Paris, Liszt returned to concert touring. Fortunately for Liszt, his father died (Liszt age 15) before too much damage was done. Like Mozart he had a stage father, who, pushed and profited from him. Oliver Hilmes does not give the reader a musical biography it is the story of how Liszt lived his life. I knew nothing about Liszt and went to You Tube to hear some of his music which I’m listening to as I write this and hear familiar refrains. Biography is my genre, and I wondered what a 19th century superstar could be. I picked this from the library shelf for its subtitle. No other Liszt biography in English is as colorful, witty, and compulsively readable, or reveals as much about the true nature of this extraordinary, outrageous talent. The author immerses the reader in the intrigues of the nineteenth-century European glitterati (including Liszt's powerful patrons, the monstrous Wagner clan) while exploring the true, complex face of the artist and the soul of his music. Whereas previous biographies have focused primarily on the composer's musical contributions, Hilmes showcases Liszt the man in all his many shades and personal reinventions: child prodigy, Romantic eccentric, fervent Catholic, actor, lothario, celebrity, businessman, genius, and extravagant show-off. Drawing on new, highly revealing documentary sources, including a veritable treasure trove of previously unexamined material on Liszt's Weimar years, best-selling author Oliver Hilmes shines a spotlight on the extraordinary life and career of this singularly dazzling musical phenomenon. A virtuoso pianist and electrifying showman, he toured extensively throughout the European continent, bringing sold-out audiences to states of ecstasy while courting scandal with his frequent womanizing. The work of Franz Liszt has been arranged by other composers and pianists, most notably Ferruccio Busoni and Marc-André Hamelin.Hungarian composer Franz Liszt (1811-1886) was an anomaly. trills with the fourth and fifth fingers starting on measure 80 and lasting for 4 bars, and fast chromatic scales starting on measure 73. The étude also involves other technical difficulties, e.g. ![]() For example, in bar 101, the left hand makes a sixteenth-note jump of just a half-step below three octaves. However, the left hand studies about four extremely large intervals, larger than those in the right hand. The two red notes are 35 half-steps apart (~46cm apart on a piano.) Fifteenth intervals are quite common in the beginning of the étude, while the sixteenth intervals appear twice, at the first thirtieth and thirty-second measures. Little time is provided for the pianist to move the hand, thus forcing the pianist to avoid tension within the muscles. Sixteenth notes are played between the two notes, and the same note is played two octaves or two octaves and a second higher with little (depending on the arrangement) no rest. The largest intervals reached by the right hand are fifteenths (two octaves) and sixteenths (two octaves and a second). As a whole, the étude can be practiced to increase dexterity and accuracy at large jumps on the piano, along with agility of the weaker fingers of the hand and muscles within the forearm and wrist. The étude is played at a gentle, brisk allegretto tempo and features constant octave hand jumps between intervals larger than one octave, sometimes even stretching for two whole octaves within the time of a sixteenth note. Incipit for "La campanella" by Franz Liszt ( Grandes études de Paganini S. |
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